Benedict's solution (Fehling's solution) is used to test for simple sugars such as glucose. It is a clear blue solution which is a combination of sodium carbonate, copper sulfate and sodium citrate. In the presence of simple sugars, the blue solution changes color to green, yellow, and brick-red, depending on the amount of sugar.
Sugar Test - Benedict's solution
- Mix small amount of each food sample with distilled water to make a test liquid.
- To a test tube, add 40 drops of liquid to be tested.
- If testing more than one liquid, label each test tube with a marker.
- Add 10 drops of Benedict's solution to each test tube. Carefully heat the test tubes by suspending in a hot water bath at about 40-50 degrees celsius for five minutes.
- Note any color change. If sugar is present solution will turn green, yellow, or brick-red, depending on sugar concentration.